I . Immunohistochemical Localization CHICK ROCKY
نویسنده
چکیده
The preparat ion of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioaUantoic membrane ( C A M ) is described. The antiC a B P appeared to be specific for the C a B P by immunodiffusion and immunoelectrophoresis. Applicat ion of the ant i -CaBP in immunofluorescence histochemistry revealed that the C a B P is present in the C A M only at developmental ages corresponding with the expression of the calcium transport function of the membrane . Fur thermore , the CaBP is localized to the ec toderm of the C A M , appears to be exposed to the entire external surface of the ec toderm, and can be shown to be associated with cells enzymatically dissociated from the C A M . These results are consistent with a functional role of the CaBP in the C A M calcium
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